![]() Phase-contrast microscopy employs special phase-contrast objectives and condensers to take advantage of refractive index variations.Īs a result, the viewed image of the specimen appears brighter or darker than its background.Īn ultraviolet microscope uses UV light to view specimens at a resolution that isn't possible with the common brightfield microscope. This contrast-enhancing optical technique makes use of the minute differences in phase to create high-contrast images of an unstained specimen. However, it’s not easy to see cell organelles using this technique.įor the purpose of viewing structures (e.g., organelles) within microscopic living cells, a phase-contrast microscope is used. This conventional technique is most suitable for observing the natural colors of the specimen. The pigmentation creates contrast which allows the viewer to see the image of the object being observed. The specimen can either be stained or colorless. The light that passes through the specimen then goes through the objective lenses and ultimately through the eyepiece. Light from the illuminator is collected by the condenser and focused at the specimen mounted on the space. The term is derived from the fact that the specimen appears darker in contrast to the bright background. Bright field microscopy is the simplest form of optical microscopy illumination techniques. Most microscopes used in classrooms are bright field microscopes. Bright Field Microscope (Best for Students) ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |